Coding

Part:BBa_K3419002:Design

Designed by: Sophia Windemuth   Group: iGEM20_Cornell   (2020-10-17)


Aspartate-semialdehyde dehydrogenase with strong promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 221
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 221
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 221
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 221
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to maximize expression of Asd in the engineered plasmid to allow plasmid retention in Asd knockout bacteria.


Source

The coding sequence comes from an Escherichia coli K12 strain, from the uniprot database (UniProtKB - P0A9Q9 (DHAS_ECOLI)). The promoter comes from Part:BBa_J23100, and the terminator comes from Part:BBa_B0015.

References